General description

Human serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:r>

Human serum that has been depleted of C1q complement component.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:r>r>CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 µl GVB++.r> Recommended volume of C1q-Dpl serum per assayr> C1qH50 units/mg purified C1q at the recommended input of C1q-Dplr> Input of purified C1q to yield 1 C1qH50:r> C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dplr> Input of C1q in NHS Complement standard to yield 1 C1qH50r> Ratior> Background A412 EA blank reading at the recommended input of C1q-Dplr>r>CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.r> CH50/ml NHS Standardr> CH50/ml C1q-Dpl reconstituted with 100 µg C1q/ml:r> Ratior>r>ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.r> APH50/ml NHS Standardr> Input of NHS Standard to yield 1 APH50r> APH50/ml C1q-Dplr> Input of C1q-Dpl to yield 1 APH50r> AP Ratio

Packaging

1 ml in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, ≤0.1% sodium azide, pH 7.4.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.

Warning

Toxicity: Standard Handling (A)